Kodi dihydrocoumarin ndi poizoni?

Dihydrocoumarin, fungo labwino, lomwe limagwiritsidwa ntchito muzakudya, limagwiritsidwanso ntchito ngati cholowa m'malo mwa coumarin, lomwe limagwiritsidwanso ntchito ngati kukoma kokongoletsa; Kirimu wosakaniza, kokonati, kukoma kwa sinamoni; Limagwiritsidwanso ntchito ngati kukoma kwa fodya.

Kodi dihydrocoumarin ndi poizoni?

Dihydrocoumarin si poizoni. Dihydrocoumarin ndi chinthu chachilengedwe chomwe chimapezeka mu chipembere chachikasu cha vanila. Chimakonzedwa ndi hydrogenation ya coumarin pamaso pa nickel catalyst pa 160-200 ℃ komanso pansi pa kupanikizika. Chingagwiritsidwenso ntchito ngati zopangira, chosungunuka mu alkaline aqueous solution kuti chipange o-hydroxyphenylpropionic acid, kusowa madzi m'thupi, komanso closed-loop.

Mkhalidwe wosungira

Potsekedwa komanso mwamdima, posungidwa pamalo ozizira komanso ouma, malo omwe ali mumtsukowo ndi ochepa momwe angathere pansi pa zilolezo zachitetezo, ndipo amadzazidwa ndi chitetezo cha nayitrogeni. Sungani m'nyumba yosungiramo zinthu yozizira komanso yopuma mpweya. Pewani moto, madzi. Iyenera kusungidwa padera ndi oxidizer, musasakanize malo osungira. Yokhala ndi mitundu yosiyanasiyana komanso kuchuluka koyenera kwa zida zozimitsira moto.

Kuphunzira mu vitro

Kuyesa kwa enzymatic mu vitro, dihydrocoumarin idayambitsa kuletsa kwa SIRT1 (IC50 ya 208μM) komwe kumadalira kuchuluka kwa shuga m'magazi. Kuchepa kwa ntchito ya SIRT1 deacetylase kudawonedwa ngakhale pa mlingo wa micromolar (85±5.8 ndi 73±13.7% pa 1.6μM ndi 8μM, motsatana). Microtubule SIRT2 deacetylase idaletsedwanso mwanjira yofanana yodalira mlingo (IC50 ya 295μM).

Pambuyo pa maola 24 akumwa mankhwala, dihydrocoumarin (1-5mM) inawonjezera poizoni m'maselo a TK6 motsatira mlingo. Dihydrocoumarin (1-5mM) inawonjezera apoptosis m'maselo a TK6 motsatira mlingo pa nthawi ya maola 6. Mlingo wa 5mM wa dihydrocoumarin unawonjezera apoptosis pa nthawi ya maola 6 mu mzere wa maselo a TK6. Pambuyo pa nthawi ya maola 24 akumwa mankhwala, dihydrocoumarin (1-5mM) inawonjezera p53 lysine 373 ndi 382 acetylation motsatira mlingo pa mzere wa maselo a TK6.