Dihydrocoumarin ndi poizoni

Dihydrocoumarin, fungo lonunkhira, lomwe limagwiritsidwa ntchito muzakudya, limagwiritsidwanso ntchito ngati choloweza m'malo mwa coumarin, chomwe chimagwiritsidwa ntchito ngati zodzikongoletsera; Sakanizani zonona, kokonati, kukoma kwa sinamoni; Amagwiritsidwanso ntchito ngati kukoma kwa fodya.

Dihydrocoumarin ndi poizoni

Dihydrocoumarin si poizoni. Dihydrocoumarin ndi mankhwala achilengedwe omwe amapezeka mu chipembere chachikasu cha vanila. Imakonzedwa ndi hydrogenation ya coumarin pamaso pa nickel chothandizira pa 160-200 ℃ komanso pansi pamavuto. Itha kugwiritsidwanso ntchito ngati zopangira, hydrolyzed mu alkaline amadzimadzi njira kubala o-hydroxyphenylpropionic acid, kuchepa madzi m'thupi, chatsekedwa kuzungulira analandira.

Mkhalidwe wosungira

Kutsekedwa ndi mdima, kusungidwa pamalo ozizira ndi owuma, malo mu mbiya ndi ochepa momwe angathere pansi pa zilolezo zachitetezo, ndipo amadzazidwa ndi chitetezo cha nayitrogeni. Sungani m'nyumba yosungiramo zinthu zoziziritsa kukhosi komanso mpweya wabwino. Khalani kutali ndi moto, madzi. Iyenera kusungidwa mosiyana ndi oxidizer, osasakaniza yosungirako. Zokhala ndi mitundu yofananira komanso kuchuluka kwa zida zozimitsa moto.

Maphunziro a in vitro

In vitro enzymatic assay, dihydrocoumarin inhibition yodalira ndende ya SIRT1 (IC50 ya 208μM). Kuchepa kwa ntchito ya SIRT1 deacetylase kudawonedwa ngakhale pamiyeso ya micromolar (85 ± 5.8 ndi 73 ± 13.7% ntchito pa 1.6μM ndi 8μM, motsatana). Microtubule SIRT2 deacetylase inaletsedwanso mofanana ndi mlingo wodalira mlingo (IC50 wa 295μM).

Pambuyo pa maola 24 akuwonekera, dihydrocoumarin (1-5mM) inachulukitsa cytotoxicity mu TK6 cell mizere m'njira yodalira mlingo. Dihydrocoumarin (1-5mM) inachulukitsa apoptosis mu mizere ya TK6 m'njira yodalira mlingo pa nthawi ya maola 6. Mlingo wa 5mM wa dihydrocoumarin unachulukitsa apoptosis pa nthawi ya maola 6 mu mzere wa cell wa TK6. Pambuyo pa nthawi ya maola 24, dihydrocoumarin (1-5mM) inachulukitsa p53 lysine 373 ndi 382 acetylation m'njira yodalira mlingo mu mzere wa selo wa TK6.